ADA2 dimers in the homozygous or heterozygous state of variants F355L, T360A, and N370K on a nondenaturing gel. (A and D) ADA2 monomer and dimer expression/secretion of HEK293T cells transfected with WT and/or ADA2 variants F355L, T360A, and N370K. Cells and supernatant were collected 48 h after transfection. The image shown represents three and two independent experiments, respectively. (B and E) Quantification of ADA2 secretion in the supernatant of HEK293T cells transfected with WT ADA2 or ADA2 variants F355L, T360A, and N370K in homozygous conditions. Bar graphs represent the percentage of ADA2 protein secretion relative to WT100% ADA2. (C and F) Quantification of ADA2 secretion of cotransfected HEK293T cells of ADA2 variants F355L, T360A, and N370K together with WT ADA2 in heterozygous conditions. Bar graphs represent the percentage of ADA2 secretion relative to WT50% ADA2. (B–F) Each bar represents the mean ± SD from three to two independent experiments, respectively. EV, empty vector. Source data are available for this figure: SourceData FS2.