Phenotypic analysis of isolated broadly neutralizing antibodies. (a) Heatmap and phylogram based on hierarchical clustering of (log10-transformed) IC₅₀ neutralization titers against a 119-heterologous virus panel. Epitope classes are shown next to the phylogram, and branch splits with >50% bootstrap support are indicated. (b) Hierarchical clustering of Pearson correlations of (log10-transformed) IC₅₀ titers for the same pseudoviruses compared across broadly neutralizing antibodies. Of the seven clusters identified, three included V2 apex lineages. All axes, the majority of needles, and the only combined VRC26 clustered in the central cluster, while the remaining needles with two combined lineages formed the cluster at the bottom. DH1020 lineage members formed their own cluster. (c) Potent rhesus and human V2 apex–targeted lineages can be divided into five neutralization groups (I–V) based on mutant virus epitope mapping. Groups III and IV have not been previously described. The envelope trimer (PDB ID 4ZMJ) highlights the location of N-linked glycan and protein residue substitutions used for V2 apex mapping. Neutralization data values from mapping experiments are provided in Table S1. (d) Correlations between plasma neutralization ID₅₀s and predicted neutralization by isolated mAbs at the specific concentration, “C,” which is provided in µg/ml. Neutralization data values for plasma and antibodies are provided in Table S1.