Figure 5.
α-CGRP is crucial in ILC2-mediated angiogenic sprouting for both mice and human. (A) Venn plot of intersecting genes from top DEGs of ILC2s and DEGs between brain ILC2s versus blood ILC2s at day 5 after MCAO. (B) Heatmap displaying the relative expression of intersecting genes at day 5 after MCAO. Genes marked by red are potentially related to brain tissue remodeling. (C) Relative transcriptional expression of Calca at three states. (D) Immunofluorescence of a brain-infiltrating ILC2 5 days after MCAO and ILC2s transfer. Scale bar, 20 µm. (E) ILC2s were co-cultured with bEnd.3 cells without direct contact, separated by a 0.4-µm membrane (created with https://BioRender.com). (F–H) Mouse-derived cell assay: In vitro mouse endothelial spheroid sprouting experiment, quantification of the number and length of sprouts per spheroid in different treatment groups (n = 5). Ri, rimegepant. Data are shown as bar plots (SD, median), analyzed by one-way ANOVA with Tukey’s multiple comparisons. *P = 0.0447; ****P < 0.0001; ns indicates P > 0.05. Scale bar, 100 µm. (I–K) Mouse-derived cell assay: In vitro mouse endothelial tube formation experiment, quantification of node number and in branch length of tubular structures different treatment groups (n = 5). Data are shown as bar plots (SD, median), analyzed by one-way ANOVA with Tukey’s multiple comparisons. **P = 0.0016; ***P = 0.0004; ****P < 0.0001; ns indicates P > 0.05. Scale bar, 250 µm. (L–N) Human-derived cell assay: In vitro HUVEC sprouting experiment, quantification of the number and length of sprouts per spheroid (n = 5). Data are shown as bar plots (SD, median), analyzed by one-way ANOVA with Tukey’s multiple comparisons. ****P < 0.0001; ns indicates P > 0.05. Scale bar, 100 µm. (O–Q) Human-derived cell assay: In vitro HUVEC tube formation experiment, quantification of node number and branch length of tubular structures (n = 5). Data are shown as bar plots (SD, median), analyzed by one-way ANOVA with Tukey’s multiple comparisons. **P = 0.0020; ****P < 0.0001; ns indicates P > 0.05. Scale bar, 250 µm.

α-CGRP is crucial in ILC2-mediated angiogenic sprouting for both mice and human. (A) Venn plot of intersecting genes from top DEGs of ILC2s and DEGs between brain ILC2s versus blood ILC2s at day 5 after MCAO. (B) Heatmap displaying the relative expression of intersecting genes at day 5 after MCAO. Genes marked by red are potentially related to brain tissue remodeling. (C) Relative transcriptional expression of Calca at three states. (D) Immunofluorescence of a brain-infiltrating ILC2 5 days after MCAO and ILC2s transfer. Scale bar, 20 µm. (E) ILC2s were co-cultured with bEnd.3 cells without direct contact, separated by a 0.4-µm membrane (created with https://BioRender.com). (F–H) Mouse-derived cell assay: In vitro mouse endothelial spheroid sprouting experiment, quantification of the number and length of sprouts per spheroid in different treatment groups (n = 5). Ri, rimegepant. Data are shown as bar plots (SD, median), analyzed by one-way ANOVA with Tukey’s multiple comparisons. *P = 0.0447; ****P < 0.0001; ns indicates P > 0.05. Scale bar, 100 µm. (I–K) Mouse-derived cell assay: In vitro mouse endothelial tube formation experiment, quantification of node number and in branch length of tubular structures different treatment groups (n = 5). Data are shown as bar plots (SD, median), analyzed by one-way ANOVA with Tukey’s multiple comparisons. **P = 0.0016; ***P = 0.0004; ****P < 0.0001; ns indicates P > 0.05. Scale bar, 250 µm. (L–N) Human-derived cell assay: In vitro HUVEC sprouting experiment, quantification of the number and length of sprouts per spheroid (n = 5). Data are shown as bar plots (SD, median), analyzed by one-way ANOVA with Tukey’s multiple comparisons. ****P < 0.0001; ns indicates P > 0.05. Scale bar, 100 µm. (O–Q) Human-derived cell assay: In vitro HUVEC tube formation experiment, quantification of node number and branch length of tubular structures (n = 5). Data are shown as bar plots (SD, median), analyzed by one-way ANOVA with Tukey’s multiple comparisons. **P = 0.0020; ****P < 0.0001; ns indicates P > 0.05. Scale bar, 250 µm.

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