Figure S8.
Validation of CDC42BPG role in pexophagy regulation using the RFP-GFP-SKL approach. (A) CDC42BPG KD efficiency was examined by WB. (B) Control and CDC42BPG KD HCT116 cells stably expressing the RFP-GFP-SKL reporter were incubated with clofibrate (1 mM) for 6 h in the presence or absence of Baf. PMP70 levels were then examined using WB. (C and D) Control and KD pexophagy reporter cells were incubated with clofibrate (1 mM, 6 h) or Torin1 (Tor, 200 nM, 24 h). The processing of RFP-GFP-SKL was analyzed by WB (C) or visualized by IF (D). Scale bars, 5 µM. Unless otherwise indicated, experiments were performed three times. Data are represented as means ± SDs, and P values were determined by two-way ANOVA. *P ≤ 0.1; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001; ns, not significant. Source data are available for this figure: SourceData FS8.

Validation of CDC42BPG role in pexophagy regulation using the RFP-GFP-SKL approach. (A) CDC42BPG KD efficiency was examined by WB. (B) Control and CDC42BPG KD HCT116 cells stably expressing the RFP-GFP-SKL reporter were incubated with clofibrate (1 mM) for 6 h in the presence or absence of Baf. PMP70 levels were then examined using WB. (C and D) Control and KD pexophagy reporter cells were incubated with clofibrate (1 mM, 6 h) or Torin1 (Tor, 200 nM, 24 h). The processing of RFP-GFP-SKL was analyzed by WB (C) or visualized by IF (D). Scale bars, 5 µM. Unless otherwise indicated, experiments were performed three times. Data are represented as means ± SDs, and P values were determined by two-way ANOVA. *P ≤ 0.1; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001; ns, not significant. Source data are available for this figure: SourceData FS8.

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