Figure S5.
Validation of CDK11A role in ER-phagy regulation using the ss-RFP-GFP-KDEL approach. (A) CDK11A KD efficiency was examined by WB. (B) Control and CDK11A KD HCT116 cells stably expressing the ss-RFP-GFP-KDEL reporter were incubated with tunicamycin (10 µg/ml) for 6 h in the presence or absence of Baf. FAM134B levels were then examined using WB. (C and D) Control and KD ER-phagy reporter cells were incubated with tunicamycin (10 µg/ml) or prolonged amino acid starvation (-AAp) for 6 h. The processing of ss-RFP-GFP-KDEL was analyzed by WB (C) or visualized by IF (D). Scale bars, 10 µM. Unless otherwise indicated, experiments were performed three times. Data are represented as means ± SDs, and P values were determined by two-way ANOVA. *P ≤ 0.1; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001; ns, not significant. Source data are available for this figure: SourceData FS5.

Validation of CDK11A role in ER-phagy regulation using the ss-RFP-GFP-KDEL approach. (A) CDK11A KD efficiency was examined by WB. (B) Control and CDK11A KD HCT116 cells stably expressing the ss-RFP-GFP-KDEL reporter were incubated with tunicamycin (10 µg/ml) for 6 h in the presence or absence of Baf. FAM134B levels were then examined using WB. (C and D) Control and KD ER-phagy reporter cells were incubated with tunicamycin (10 µg/ml) or prolonged amino acid starvation (-AAp) for 6 h. The processing of ss-RFP-GFP-KDEL was analyzed by WB (C) or visualized by IF (D). Scale bars, 10 µM. Unless otherwise indicated, experiments were performed three times. Data are represented as means ± SDs, and P values were determined by two-way ANOVA. *P ≤ 0.1; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001; ns, not significant. Source data are available for this figure: SourceData FS5.

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