Figure 4.
Reactivity of IgG in FI-deficient patient’s serum with bacterial lysates of N. gonorrhoeae. (A) Reactivity of the FI-def. patient’s serum against N. gonorrhoeae Rmp. Lysates prepared from N. gonorrhoeae strain FA1090 and its Rmp deletion mutant (FA1090 ΔRmp) were separated on a 4–12% Bis-Tris gel using MES SDS running buffer and transferred to a membrane by western blotting. The blot was divided into four sections and probed with the patient’s serum (1:500 dilution) or with anti-Rmp antiserum, as indicated beneath each section. IgG-reactive bands were disclosed with anti-human IgG or anti-mouse IgG conjugated to ALP. The two remaining sections were probed with the ALP conjugates alone, which served as background controls. All blots were developed for the same time. Black asterisks denote the location of Rmp. (B) IgG in the patient’s serum reacts against the gonococcal lipoproteins H.8 and Laz. Lysates of three N. gonorrhoeae strains (FA1090, WR220, and 15253) were separated on a 12% Bis-Tris gel, transferred to a membrane by western blotting, and probed with either the patient’s serum or anti-H.8/Laz mAb 2-8C-4-1. The locations of H.8 and Laz are indicated by red and blue asterisks, respectively. (C) Reactivity of IgG in patient’s serum with N. meningitidis H.8 and Laz lipoproteins. Western blotting of lysates of N. meningitidis strain MC58 and its isogenic H.8 and Laz deletion mutants was performed as described in B. Samples were run until the 15 kDa marker was at the bottom of the gel to better separate bands in close proximity to the H.8 band. The positions of H.8 and Laz are indicated by red and blue asterisks, respectively. A band that migrates just above H.8 is seen in the blot probed with the patient’s serum in the MC58 ΔH.8 lane. MW, molecular weight marker (kDa); def., deficient; mAb, monoclonal antibody. Source data are available for this figure: SourceData F4.

Reactivity of IgG in FI-deficient patient’s serum with bacterial lysates of N. gonorrhoeae. (A) Reactivity of the FI-def. patient’s serum against N. gonorrhoeae Rmp. Lysates prepared from N. gonorrhoeae strain FA1090 and its Rmp deletion mutant (FA1090 ΔRmp) were separated on a 4–12% Bis-Tris gel using MES SDS running buffer and transferred to a membrane by western blotting. The blot was divided into four sections and probed with the patient’s serum (1:500 dilution) or with anti-Rmp antiserum, as indicated beneath each section. IgG-reactive bands were disclosed with anti-human IgG or anti-mouse IgG conjugated to ALP. The two remaining sections were probed with the ALP conjugates alone, which served as background controls. All blots were developed for the same time. Black asterisks denote the location of Rmp. (B) IgG in the patient’s serum reacts against the gonococcal lipoproteins H.8 and Laz. Lysates of three N. gonorrhoeae strains (FA1090, WR220, and 15253) were separated on a 12% Bis-Tris gel, transferred to a membrane by western blotting, and probed with either the patient’s serum or anti-H.8/Laz mAb 2-8C-4-1. The locations of H.8 and Laz are indicated by red and blue asterisks, respectively. (C) Reactivity of IgG in patient’s serum with N. meningitidis H.8 and Laz lipoproteins. Western blotting of lysates of N. meningitidis strain MC58 and its isogenic H.8 and Laz deletion mutants was performed as described in B. Samples were run until the 15 kDa marker was at the bottom of the gel to better separate bands in close proximity to the H.8 band. The positions of H.8 and Laz are indicated by red and blue asterisks, respectively. A band that migrates just above H.8 is seen in the blot probed with the patient’s serum in the MC58 ΔH.8 lane. MW, molecular weight marker (kDa); def., deficient; mAb, monoclonal antibody. Source data are available for this figure: SourceData F4.

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