Autophagy in Mkt1 and Pbp1 truncation strains during growth in minimal respiratory medium. (A) Schematic representation of Flag-tagged Pbp1 truncation strains tested for their capacity to undergo autophagy during growth in SL medium. (B) GFP cleavage assay showing Pbp1Δ491-570 and Pbp1Δ491-513 cells had disrupted mitophagy following incubation in SL medium for 6 h. (C) GFP cleavage assay showing Pbp1Δ500-514 cells had disrupted mitophagy following incubation in SL medium for 6 h. Note: residual free GFP in Pbp1Δ500-514 cells was detected, which may reflect continued TORC1 repression. (D) GFP cleavage assay showing Mkt1 truncation mutants had disrupted mitophagy following incubation in SL medium for 6 h. * indicates a nonspecific band. Source data are available for this figure: SourceData FS5.