Assessment of Mkt1/Pbp1 complex and Puf3 localization to polysomes. (A) Polysome profile of WT cells collected 3 h following switch from YPD to YPL medium and associated western blot for Pbp1-Flag, Mkt1-HA, Puf3-myc, and Pab1 in collected fractions. Note: Mkt1-HA, Pbp1-Flag, and Puf3-myc were present in polysome fractions. (B) Polysome profile of mkt1∆ cells collected 3 h following switch from YPD to YPL medium and associated western blot for Pbp1-Flag, Puf3-HA, and Pab1 in collected fractions. Note: Pbp1-Flag and Puf3-HA were present in polysome fractions. (C) Polysome profile of Pbp1-4A cells collected 3 h following switch from YPD to YPL medium and associated western blot for Pbp1-Flag, Mkt1-HA, and Pab1 in collected fractions. Note: Mkt1-HA was less present in polysome fractions. Source data are available for this figure: SourceData FS4.