Interaction and bioinformatic analyses of S. cerevisiae Mkt1. (A) AlphaFold protein structure prediction of S. cerevisiae Mkt1 (AF-P40850-F1-v4) and schematic depicting Mkt1 protein domains. (B) Schematic representation of Mkt1 truncation mutants assayed for their ability to interact with Pbp1 in C. The dotted lines represent the deleted regions. (C) co-IP testing interactions between Flag-tagged Mkt1 truncation mutants and Pbp1-HA. Cells were collected from YPL cultures grown to log phase. All Mkt1 mutants tested had disrupted interactions with Pbp1-HA and reduced Pbp1-HA protein abundance. Source data are available for this figure: SourceData FS2.