Mkt1 and Pbp1 form a complex under fermentative and respiratory conditions. (A) Silver stain of immunoprecipitated Flag-tagged Mkt1 and Pbp1 protein samples used in IP/MS experiment to identify protein interactors. Cells were collected from YPD cultures in log phase and 3 h following switch to YPL medium. (B) Top four most abundant proteins in each IP/MS sample from A. (C) IP/MS experiment to identify protein bands present on silver stain gel of Mkt1-Flag and Pbp1-Flag IP samples. Length of gel sections cut and sent for protein identification is depicted. Cells were collected from YPL cultures grown to log phase. (D) co-IP experiment showing Mkt1–Pbp1 interaction is not dependent on RNA. IP samples were incubated with RNase If for 15 min at 37°C. Cells were collected from YPL cultures grown to log phase. Source data are available for this figure: SourceData F1.
Figure 1.

Mkt1 and Pbp1 form a complex under fermentative and respiratory conditions. (A) Silver stain of immunoprecipitated Flag-tagged Mkt1 and Pbp1 protein samples used in IP/MS experiment to identify protein interactors. Cells were collected from YPD cultures in log phase and 3 h following switch to YPL medium. (B) Top four most abundant proteins in each IP/MS sample from A. (C) IP/MS experiment to identify protein bands present on silver stain gel of Mkt1-Flag and Pbp1-Flag IP samples. Length of gel sections cut and sent for protein identification is depicted. Cells were collected from YPL cultures grown to log phase. (D) co-IP experiment showing Mkt1–Pbp1 interaction is not dependent on RNA. IP samples were incubated with RNase If for 15 min at 37°C. Cells were collected from YPL cultures grown to log phase. Source data are available for this figure: SourceData F1.

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