The induction of endogenous TDP-MP20 expression by hnRNP K correlates with that of hnRNP A1. (A) Schematic representation of hnRNP K with KH or KI/KNS domain deletion (Del KH, Del KI/KNS) or with KH domain mutations (KHmt). (B and C) RT-PCR images of TARDBP splicing (B) or RT-qPCR analysis of TDP-FL and MP20 (127) mRNA expression levels (C) in HEK293T cells overexpressing hnRNP K deletion mutants. (D) WB of HEK293T cells overexpressing deletion mutants of hnRNP K. (E and F) RT-PCR images of TARDBP splicing (E) or RT-qPCR analysis of TDP-FL and MP20 (127) mRNA expression levels (F) in HEK293T cells overexpressing KHmts. In C and F, data are normalized to ACTB. Statistical analyses were performed using one-way ANOVA followed by Tukey’s test. (G) WB of HEK293T cells overexpressing KHmts. In D and G, quantification of the relative expression of FL-endo, SVs-endo, MP20-endo, hnRNP A1, and hnRNP A2/B1 is shown, respectively. Data are normalized to β-actin. Statistical analyses were performed using one-way ANOVA followed by Tukey’s test (n = 3 for each group). All graphs show the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 compared with the NoTF Ctr group, and #P < 0.05, ##P < 0.01, ###P < 0.001, and ####P < 0.0001 compared with the WT hnRNP K group. NoTF Ctr, nontransfected control; NLS, nuclear localization signal. Source data are available for this figure: SourceData F8.