Responsible region of hnRNP A1 on TARDBP exon 6 splicing is not found in TARDBP intron 5 and cryptic intron 6. (A) Schematic representation of a mini-gene containing exon 6 (1–2,100) (Ex6) or exon 5 to exon 6 (Ex5-Int5-Ex6) of TARDBP. A potential hnRNP A1 consensus site is indicated in the Ex5-Int5-Ex6 mini-gene. Orange or blue lines show alternative donor (D) or acceptor (A) sites, respectively, and magenta lines indicate the stop codon of FL. (B) RT-PCR images showing the effect of hnRNP A1 on splicing of the mini-gene (Ex5-Int5-Ex6) with deletion of the potential hnRNP A1 consensus sequence in HEK293T cells. (C and E) Schematic representation of deletion mutants of the TARDBP mini-gene (Ex5-Int5-Ex6). Green-filled box indicates polypyrimidine tract (PPT). (D and F) RT-PCR images of HEK293T cells coexpressing TARDBP mini-genes (Ex5-Int5-Ex6) with or without hnRNP A1. Source data are available for this figure: SourceData FS3.
Figure S3.

Responsible region of hnRNP A1 on TARDBP exon 6 splicing is not found in TARDBP intron 5 and cryptic intron 6. (A) Schematic representation of a mini-gene containing exon 6 (1–2,100) (Ex6) or exon 5 to exon 6 (Ex5-Int5-Ex6) of TARDBP. A potential hnRNP A1 consensus site is indicated in the Ex5-Int5-Ex6 mini-gene. Orange or blue lines show alternative donor (D) or acceptor (A) sites, respectively, and magenta lines indicate the stop codon of FL. (B) RT-PCR images showing the effect of hnRNP A1 on splicing of the mini-gene (Ex5-Int5-Ex6) with deletion of the potential hnRNP A1 consensus sequence in HEK293T cells. (C and E) Schematic representation of deletion mutants of the TARDBP mini-gene (Ex5-Int5-Ex6). Green-filled box indicates polypyrimidine tract (PPT). (D and F) RT-PCR images of HEK293T cells coexpressing TARDBP mini-genes (Ex5-Int5-Ex6) with or without hnRNP A1. Source data are available for this figure: SourceData FS3.

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