Negative stain EM shows diverse caveolins assemble into disk-shaped complexes. (A)H. sapiens Cav1, (B)S. purpuratus caveolin, (C)A. queenslandica caveolin, and (D)S. rosetta caveolin. In each panel, surface-filling models for the cryo-EM structure or AF2.2 11-mer structures are shown on the left, representative images of negatively stained caveolin complexes are shown in the middle, and representative 2D averages of caveolins are shown on the right. The number of particles found in each class average is shown in the bottom right. Scale bar, 30 nm. (E) Western blot of Blue native gel with bracket marking the position of 8S-like complexes. Molecular weight markers are indicated. Source data are available for this figure: SourceData F6.
Figure 6.

Negative stain EM shows diverse caveolins assemble into disk-shaped complexes. (A) H. sapiens Cav1, (B)S. purpuratus caveolin, (C)A. queenslandica caveolin, and (D)S. rosetta caveolin. In each panel, surface-filling models for the cryo-EM structure or AF2.2 11-mer structures are shown on the left, representative images of negatively stained caveolin complexes are shown in the middle, and representative 2D averages of caveolins are shown on the right. The number of particles found in each class average is shown in the bottom right. Scale bar, 30 nm. (E) Western blot of Blue native gel with bracket marking the position of 8S-like complexes. Molecular weight markers are indicated. Source data are available for this figure: SourceData F6.

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