Ligand–receptor analysis of IL-6 cytokine family members identify oncostatin M signaling between myeloid cells and astrocytes/fibroblasts. (A) Dot plot of expression of IL-6 family member ligands (left) and their receptors (right) in previously identified cell subtypes across injury time points. In each panel, dotted lines demarcate myeloid (left column), vascular (middle column), or macroglial (right column) subtypes. Color of dots represents z-scored expression, and size of dots represents percentage of cells with at least one UMI detected per gene. Gene members of the IL-6 cytokine family that were not expressed in at least 10% of any subtype at any time point were excluded. Inset stars indicate statistically significant greater expression compared with all other cells combined (adjusted P value < 10−10; Wilcoxon rank-sum with Bonferroni correction; see Materials and methods). (B) Dot plot of ligand–receptor interaction scores of IL-6 family member signaling shows highest predicted interactions between myeloid cells and astrocytes/fibroblasts via onctostatin M signaling. Size of dot represents –log10(P value), and color represents interaction score where dark red colors indicate greater predicted interactions. Specific IL-6 family ligand–receptor pairs are listed along left axis. Ligand-expressing cells are listed across top axis. Receptor-expressing subtypes are listed across bottom axis. Select subtypes with greatest number of significant scores are shown. A, arterial; Astroepen., astroependymal; C, capillary; Div, dividing; Homeo, homeostatic; Inflamm, inflammatory; Mac, macrophage; OPC, oligodendrocyte progenitor cell; V, venous.