Molecular profile of macroglia cells after SCI. (A) Individual UMAP representations of the expression pattern of previously described marker genes used to annotate each macroglia cluster. (B) In situ hybridization (Crym in white) and immunohistological (GFAP in green) validation of astroependymal cell formation in response to injury. Boxes depict the location of higher magnification images shown below. Images in g, i, k, and m were taken from the central canal. Images shown in h, j, l, and n were taken outside of the central canal. White arrowheads denote DAPI⁺/Crym⁺ cells, and red arrowheads denote DAPI⁺/Crym⁺/GFAP⁺ cells. DAPI is in blue labeling cell nuclei. GFAP-negative region in injured tissue represents the lesion. (C) Quantification of Crym⁺ astroependymal cells in the central canal as defined by the percentage of DAPI⁺ nuclei that colocalize with Crym⁺ in situ signal surrounding the central canal. *, P < 0.05 compared with all other groups using one-way ANOVA with Tukey’s post-test. n = 3 biological replicates per time point.