Distribution of clusters in HEK293T cells co-expressing BK and GluN1-GluN2B ^WT or BK with GluN1-GluN2B ^V618G . Representative cluster analysis data with radius cutoff set to 20 nm (see Fig. S1 for a complete description of analyses and data). (A) Cumulative probability analysis of heteroclusters size distribution in cells co-expressing BK and either GluN1-GluN2B^WT or GluN1-GluN2B^V618G ****P < 0.0001 (Kolmogorov–Smirnov test; D = 0.1183). (B) Percentage of cluster types in cells co-expressing BK plus either GluN1-GluN2B^WT (black) or GluN1-GluN2B^V618G (orange).