Membrane abundance of different NMDAR combinations measured with TIRFM. (A) Representative TIRFM images of HEK293T cells transiently transfected with NMDAR containing combinations of GluN1a and either WT- or mutant-GluN2B subunits. Scale bar = 5 μm. (B) Quantitative analysis of TIRFM imaging from cells expressing heteromers of GluN1a and the indicated GluN2B variants, normalized to the fluorescence levels of GluN1-GluN2BWT (black circles). Data represent mean ± SEM (minimum n = 30 cell counts per condition and experiment; three independent experiments). ****, P < 0.0001 (Kruskal–Wallis/Dunn’s test).
Figure 6.

Membrane abundance of different NMDAR combinations measured with TIRFM. (A) Representative TIRFM images of HEK293T cells transiently transfected with NMDAR containing combinations of GluN1a and either WT- or mutant-GluN2B subunits. Scale bar = 5 μm. (B) Quantitative analysis of TIRFM imaging from cells expressing heteromers of GluN1a and the indicated GluN2B variants, normalized to the fluorescence levels of GluN1-GluN2BWT (black circles). Data represent mean ± SEM (minimum n = 30 cell counts per condition and experiment; three independent experiments). ****, P < 0.0001 (Kruskal–Wallis/Dunn’s test).

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