Related to Fig. 7 . Neutrophils shape lesions throughout infection. (A) Pearson correlation coefficients of the indicated cell populations within lesion microenvironments. (B) Heatmap showing cellular composition of clustered microenvironments and percent area of lesion comprised by each microenvironment, uninvolved regions not included, corresponding to Fig. 7 E. (C) Density of MDCs and neutrophils within lesions. (D) Representative confocal microscopy image showing CXCL2 staining (magenta). Scale bar: 50 μm. (E) Quantitative imaging determination of CD11b MFI within neutrophils. (F) Heatmap showing cellular composition of clustered microenvironments and percent area of lesion comprised by each microenvironment, uninvolved regions not included, corresponding to Fig. 7 J. (G) Confocal microscopy image of one small necrotic lesion with low antigen abundance, identified following late Ly6G depletion. Single-group comparisons by unpaired t test. Scale bar: 250 μm. (H) Quantitative imaging determination of CD4⁺ T cell and pS6+ CD4⁺ T cell density within lesions. (I) Quantitative imaging determination of MDC density and MHCII positivity within lesions. Single-group comparisons by unpaired t test. *P < 0.05, **P < 0.01, and ns, P ≧ 0.05.