Neutrophils shape lesions throughout infection. (A) Experimental outline for B–D. Mice received CD aerosol infection, then were administered αLy6G-depleting antibody or isotype from d7–d15, lungs were taken on d15. (B) Pulmonary bacterial burdens. (C) Representative confocal images and quantification showing increased MHCII+ in MDCs following αLy6G administration. Scale bar: 50 μm. (D) Representative confocal images and quantification showing increased pS6+ T cells following αLy6G administration. Scale bar: 50 μm. (E) Experimental outline for F–I. Mice received CD aerosol infection, then were administered αLy6G-depleting antibody or isotype from d7–d15, lungs were taken on d43. (F) Representative confocal images showing abrogation of necrosis with “early” αLy6G administration. Scale bar: 250 μm. (G) Pulmonary bacterial burdens. (H) Percent of lesion comprised by necrotic region (pink). (I) Percent of lesion comprised by microenvironments with high neutrophil density. (J) Proportion of lesion neutrophils that express CXCL2. (K) Experimental outline for L–N. Mice received CD aerosol infection, then were administered αLy6G-depleting antibody or isotype from d28–d49, lungs were taken on d50. (L) Representative confocal images showing decreased necrosis with “late” αLy6G administration. Scale bar: 250 μm. (M) Percent of lung area comprised by necrotic region (pink). (N) Pulmonary bacterial burdens. Single-group comparisons by unpaired t test (B–D, G, and N) or Mann–Whitney U test (H–J and M). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, and ns, P ≥ 0.05. Points represent individual mice. Data are each representative of two independent experiments with at least four mice per group. See also Fig. S5.