Neutrophils drive lesion necrosis. (A) Experimental outline for A–H. Mice received CD aerosol infection, then were administered αLy6G-depleting antibody or isotype from d7–d28, lungs were taken on d29. (B) Representative confocal images showing abrogation of necrosis with αLy6G administration. Scale bar: 150 μm. (C) Pulmonary bacterial burdens. (D) Representative map showing 50 µm² neighborhoods, color-coded microenvironment, and heatmap showing cellular composition of clustered microenvironments Scale bar: 1 mm. (E) Percent area of lesion comprised by each microenvironment. Uninvolved regions (gray) not included. (F) Percent lesion (any color) of total lung area. (G) Percent of lesion comprised by necrotic region (pink). (H) Representative confocal images and quantification showing increased pS6+ T cells following αLy6G administration. Scale bar: 50 μm. (I) Representative confocal images and quantification showing increased MHCII+ in MDCs following αLy6G administration. Scale bar: 50 μm. Single-group comparisons by unpaired t test (C and F) or Mann–Whitney U test (G–I). *P < 0.05, **P < 0.01, ***P < 0.001, and ns, P ≥ 0.05. Points represent individual mice. Data are each representative of three independent experiments with at least four mice per group. See also Fig. S4.