CD4 T cell and neutrophil deplation shape lesion composition. (A and B) Related to Fig. 5. CD4 T cells are required for CoMtb-mediated protection from lesion necrosis. (A) Representative flow plots and CD4 T cell enumeration following αCD4-depleting antibody administration. (B) Representative flow plots and neutrophil enumeration following αCD4-depleting antibody administration. Single-group comparisons by unpaired t test. (C–F) Related to Fig. 6: Neutrophils drive lesion necrosis. (C) Representative flow plots and neutrophil enumeration following αLy6G-depleting antibody administration. Also demonstrates concordance of CD177 and Ly6G in an Mtb-infected lung. (D) Validation of necrotic regions with nuclear debris objects. Scale bar: 1 mm. (E) Quantitative imaging determination of CD4⁺ T cell density within lesions. (F) Quantitative imaging determination of MDC density within lesions. Single-group comparisons by unpaired t test. **P < 0.01, ***P < 0.001, ****P < 0.0001, and ns, P ≧ 0.05.