CoMtb shapes early tuberculous lesion cellularity and organization. d17 after CD infection, n = 5 per group. (A) Representative confocal images showing lesions and zoom-ins highlighting T cells, MDCs, and neutrophils. Scale bar: 200 μm; zoom: 50 μm. 1–2 color images from T cell regions are shown in Fig. S3 D. (B) Relative cellular density of specified cell types within lesions as determined by histo-cytometry. (C) Ratio of PPD+ neutrophils to PPD+ macrophages. (D) PPD MFI of PPD+ neutrophils. (E) Pearson correlation coefficients of the indicated cell populations within microenvironments. (F) Confocal image and spots/neighborhood of p120 staining. Scale bar: 50 μm. Single-group comparisons by unpaired t test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, and ns, P ≥ 0.05. Correlations by Pearson’s correlation test. Points represent individual lesions. Data are representative of two independent experiments with at least four mice per group. See also Fig. S3.