CoMtb accelerates T cell and MDC activation, blunts neutrophil responses. Multiple time points after CD infection. (A) GSEA analysis showing pathways enriched following aerosol infection at days 10, 17, and 34 p.i., in the setting of primary infection and CoMtb. (B) Predicted strength of selected T cell to myeloid cell signaling interactions quantified using CellChat. (C) Predicted strength of significant neutrophil to MDC interactions using CellChat. (D) Predicted strength of significant neutrophil to T cell interactions using CellChat. (E) Predicted strength of significant neutrophil to neutrophil chemotactic interactions using CellChat. (F) Levels of IFN and CXCL2, measured by Luminex. False discovery rate-adjusted P values determined using the R fgsea package. Dots in B–E indicate strength is significantly higher compared with a null distribution (i.e., CellChat-reported P value <0.05). *P < 0.05, and ns, P ≥ 0.05. Single-group comparisons in D and F by t test. Data are representative of one (A–E) or two independent experiments (F) experiments with at least four mice per group. See also Fig. S3. FDR, false discovery rate.