Related to Fig. 1 . Pre-existing immunity abrogates the formation of necrotic granulomas: (A) Lesion zoom-ins from Fig. 1 A. Scale bar: 1 mm. (B) PCA loadings from Fig. 1 B. (C) Representative confocal images showing necrotic debris in center of primary necrotic lesion and intact nuclei surrounded by p120 staining within lesion formed in setting of CoMtb. Scale bar: 50 μm. (D) Representative confocal images of markers used for analysis in Fig. 1. Scale bar: 25 μm. (E) Histo-cytometry gating scheme to determine cell types for analysis in Fig. 1, G–K. Scale bar: 500 μm; zoom: 100 μm.