AIVE for the analysis of the mitochondrial interactome. (A–F) Quantification of the closest approach distance separating each individual mitochondrion from (A) the rest of the mitochondrial network, (B) the ER, (C) EEs, (D) LEs, (E) LDs, or (F) cytosolic vesicles. (G) Average percentage of all mitochondria detected within 35 nm of the indicated organelle per set. (H–J) 3D rendering of distance-mapped mitochondrial surfaces from a subregion in set #1. The mitochondrial surfaces are shaded with a colorimetric scale indicating surface distances from (H) other mitochondria, (I) the ER, or (J) cytosolic vesicles. (K–M) Surface area histograms for the mitochondria located within 100 nm of the indicated organelle, depicting the average percentage of a mitochondrial surface located within indicated distance of (K) other mitochondria, (L) the ER, or (M) cytosolic vesicles (arrowheads in K and L indicate the anomalous peak, explained in Fig. S5, A–D). Plot markers in A–F indicate value for individual mitochondria. The interquartile range is indicated by the box, the median is indicated by horizontal lines, and the minimum and maximum are indicated by whiskers. Data in G are mean ± SD calculated for the averages in each dataset. Histogram data in K–L are mean surface area percentages binned into 5 nm-distance brackets relative to the target organelle; mean values for each set are also shown. Scale bars; H–J, 200 nm. mt, mitochondria.