The morphometric analysis of individual mitochondria via AIVE. (A–G) Mitochondria of interest that were detected in the AIVE-processed datasets are shown in Fig. 4, showing the membranes of each mitochondrion (red) alongside its matrix (blue) and the binary skeleton calculated for its bulk morphology (black; longest-optimal path endings shown in yellow); these include (A) the smallest mitochondrion, (B) the largest mitochondrion, (C) a nanotunnelling mitochondrion, (D) the most elongated mitochondrion, (E) the mitochondrion with the longest continuous tubule, (F) the most spherical mitochondrion, and (G) the mitochondrion closest to the average values detected by all morphological metrics. For animations of these data, see Video 4 (for A and B), Video 6 (for C, F, and G), and Video 5 (for D and E). (H–L) Quantitative analysis of key morphological metrics of all mitochondria in each set: (H) box whisker chart for the total volume of each mitochondrion, (I) a scatter chart showing the relationship between the volume of membrane (x axis) and volume of matrix (y axis) in each mitochondrion, with (J–L) box whisker charts showing additional metrics for (J) mitochondrial sphericity, (K) mitochondrial length, and (L) mitochondrial elongation. See Video 4. Plot markers indicate value for individual mitochondria. Hollow plot markers indicate mitochondria that were intersected by the dataset boundary; these mitochondria were excluded from analyses sensitive to incomplete mitochondria (Fig. 5, A, E, D, G, and J–L). The interquartile range for mitochondrial population is indicated by the box, the median is indicated by the horizontal lines, and the minimum and maximum are indicated by whiskers. Scale bars; A–G, markers on the scale grid are 1,000-nm apart.