The compositional analysis of membranes in bulk cellular volumes via AIVE. (A–D) 3D renderings of the three FIB-SEM datasets processed via AIVE for quantitative analysis, showing (A) the test data used in Figs. 1, 2, and 3 alongside overviews of (B) the dataset it originated from (set #1; leading lines indicate location), and (C and D) two additional datasets processed via AIVE (set #2 and set #3, respectively). All three overviews are shown at the same scale (see Video 3 for animated cutaways of the data). (E–G) Compositional analysis of membrane volumes belonging to non-nuclear cytoplasmic organelles in (E) set #1, (F) set #2, and (G) set #3. See Video 3 for animated cutaways of overview data. Scale bars; A, the bounding box is 2,400-nm wide; B–D, markers on the scale grid for overviews are 1,000-nm apart.