Figure 6.
UBXD8 localizes to peroxisomes. (A) Airyscan images of COS-7 cells transfected with GFP-UBXD8 and RFP-SKL and stained with BODIPY (665/676) to label lipid droplets. (B) Quantification of RFP-SKL colocalization to GFP-UBXD8 by Mander’s colocalization coefficient. Error bars indicate the mean ± SE, COS-7 cells (n = 17) and HeLa cells (n = 8). The RFP channel is rotated 90° right to quantify random colocalization. **P < 0.01, ***P < 0.001, unpaired t test. The scale bar is 2 μM. (C and D) Identical to A and B except in HeLa cells. (E) Airyscan images of HeLa cells transfected with GFP-SKL and stained for endogenous UBXD8. (F) Quantification of GFP-SKL colocalization to endogenous UBXD8 by Mander’s colocalization coefficient. Error bars indicate the mean ± SE, n = 13. The GFP channel is rotated 90° right to quantify random colocalization. ****P < 0.0001, unpaired t test. The scale bar is 5 μM.

UBXD8 localizes to peroxisomes. (A) Airyscan images of COS-7 cells transfected with GFP-UBXD8 and RFP-SKL and stained with BODIPY (665/676) to label lipid droplets. (B) Quantification of RFP-SKL colocalization to GFP-UBXD8 by Mander’s colocalization coefficient. Error bars indicate the mean ± SE, COS-7 cells (n = 17) and HeLa cells (n = 8). The RFP channel is rotated 90° right to quantify random colocalization. **P < 0.01, ***P < 0.001, unpaired t test. The scale bar is 2 μM. (C and D) Identical to A and B except in HeLa cells. (E) Airyscan images of HeLa cells transfected with GFP-SKL and stained for endogenous UBXD8. (F) Quantification of GFP-SKL colocalization to endogenous UBXD8 by Mander’s colocalization coefficient. Error bars indicate the mean ± SE, n = 13. The GFP channel is rotated 90° right to quantify random colocalization. ****P < 0.0001, unpaired t test. The scale bar is 5 μM.

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