Depletion of UBXD8 results in an increase of VLCFAs and a loss in catalase activity. (A) Volcano plot of the total CE and triacylglycerol species identified using lipidomics of whole-cell extracts of HEK293T cells (−log₁₀-transformed P value versus the log₂-transformed ratio of UBXD8 KO to WT). VLCFA species indicated for CE (orange) and TG (dark blue). This dataset has been previously published in Ganji et al. (2023) and is reanalyzed here. (B) VLCFA species indicated for PS (green), PE (red), and PC (violet). Lipids were measured by LC-MS/MS following normalization by total protein amount (n ≥ 3 biologically independent experiments were performed, each with duplicate samples). This dataset has been previously published (Ganji et al., 2023) and is reanalyzed here. (C) Catalase activity in WT and UBXD8 KO cells was quantified using a commercial kit. N = 3 independent experiments. **P < 0.0001, unpaired t test. (D) Immunoblots of catalase levels in whole-cell lysates of HeLa WT and UBXD8 KO cells. (E) Quantification of catalase levels in D. N = 3 independent experiments. ns: not significant, unpaired t test. Source data are available for this figure: SourceData F5.