Figure 4.
Role of functional domains in UBXD8 in maintaining peroxisome abundance. (A) Top: schematic of domains in UBXD8. Bottom: complementation of UBXD8 KO cells with WT UBXD8-HA, UBXD8-UBA*-HA, UBXD8-ΔUAS-HA, or UBXD8-UBX*-HA. Cells were stained for peroxisomes using peroxisomal matrix marker catalase. (B) Quantification of average peroxisomes per cell from images (A). Peroxisome numbers were quantified in cells expressing low levels of HA-tagged UBXD8 constructs only. At least 100 cells were analyzed in N = 3 independent experiments. The violin plot shows median and 95% confidence intervals. ****P < 0.0001, two-way ANOVA with Tukey’s multiple comparisons test. (C) Immunoblots of constructs transfected. The scale bar is 10 μM. Source data are available for this figure: SourceData F4.

Role of functional domains in UBXD8 in maintaining peroxisome abundance. (A) Top: schematic of domains in UBXD8. Bottom: complementation of UBXD8 KO cells with WT UBXD8-HA, UBXD8-UBA*-HA, UBXD8-ΔUAS-HA, or UBXD8-UBX*-HA. Cells were stained for peroxisomes using peroxisomal matrix marker catalase. (B) Quantification of average peroxisomes per cell from images (A). Peroxisome numbers were quantified in cells expressing low levels of HA-tagged UBXD8 constructs only. At least 100 cells were analyzed in N = 3 independent experiments. The violin plot shows median and 95% confidence intervals. ****P < 0.0001, two-way ANOVA with Tukey’s multiple comparisons test. (C) Immunoblots of constructs transfected. The scale bar is 10 μM. Source data are available for this figure: SourceData F4.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal