Role of ER stress and UBXD8 HP domain in regulation of peroxisomes. (A) HeLa WT and UBXD8 KO cells were treated with tunicamycin (2.5 μM for 4 h) and stained for catalase. (B) Quantification of peroxisomes per cell from A. 50–100 cells were analyzed in N = 3 independent experiments. The violin plot shows median and 95% confidence intervals. ns: not significant, ****P < 0.0001, two-way ANOVA with Dunnett’s multiple comparisons test. (C) Rescue of peroxisome number in UBXD8 KO cells transfected with either UBXD8-HA or UBXD8-ΔHP. Cells were stained for peroxisomes using peroxisomal matrix marker catalase. (D) Quantification of average peroxisomes per cell from C. Peroxisome numbers were quantified in cells expressing tagged UBXD8 constructs only. At least 100 cells were analyzed in N = 3 independent experiments. The violin plot shows median and 95% confidence intervals. ****P < 0.0001, two-way ANOVA with Tukey’s multiple comparisons test. (E) Immunoblots of constructs transfected. The scale bar is 10 μM. Source data are available for this figure: SourceData FS3.