UBXD8 deletion leads to loss of peroxisomes in HeLa and HEK293T cell lines. (A) HeLa WT and UBXD8 KO cells stained for peroxisomes using peroxisomal matrix protein catalase. (B) Peroxisome numbers in WT and UBXD8 KO cells that have either no peroxisomes or <10 peroxisomes per cell as measured by catalase. ns: not significant, **P < 0.001, unpaired t test. (C) HeLa WT and UBXD8 KO cells stained for peroxisomes using PMP70. (D) Peroxisome numbers in WT and UBXD8 KO cells that have either no peroxisomes or <10 peroxisomes per cell as measured by PMP70. ns: not significant, *P < 0.05, unpaired t test. (E) HEK293T WT and UBXD8 KO cells stained for peroxisomes using peroxisomal matrix protein catalase. (F) Quantification of peroxisomes per cell and peroxisome size from E. 50–100 cells were analyzed in N = 3 independent experiments. The violin plot shows median and 95% confidence intervals. ****P < 0.0001, N = 3, unpaired t test. (G and H) Same as (E and F) but stained for PMP70. The scale bar is 10 μM (A, C, E, and G).