Additional data related to Fig. 3 . (A) Expression of IP3Rs in MDA-MB-231 and LM2-4 cells. (A-left) Representative images of WT and dKO migrating cells under normal conditions or low Ca²⁺, as indicated. (A-right) Quantification of the number of migrating cells from each group was normalized to that of control (WT) cells. Scale bar = 350 μm. (B and C) Representative images (left) and quantitation (right) of WB analysis (n = 3–4) of lysates prepared from the indicated MDA-MB-231 (A) or LM2-4 (B) cells using antibodies for IP3R1, IP3R2, IP3R3, or tubulin, as indicated. (D) Migration analysis of WT, S1KO, S2KO, or dKO cells treated with the indicated ITPR siRNAs. Bars show the mean ± SEM. Statistics: one-way ANOVA with Tukey’s post hoc test; *P < 0.05, **P < 0.01, ***P < 0.001. Source data are available for this figure: SourceData FS3.