Expression of EMT markers and NFAT1 in MDA-MB-231 and MCF-7 cells. (A–D) Representative images (left) and quantitation (right) of WB analysis (n = 3–4) of lysates prepared from the indicated MDA-MB-231 or MCF-7 cells using antibodies for vimentin and actin (A), NFAT1 and GAPDH (B), Snail and tubulin (C), or E-cadherin and actin (D). (E) Quantification of total F-actin content in the indicated MDA-MB-231 cells (see Materials and methods). Bars show the mean ± SEM. Source data are available for this figure: SourceData FS2.