Additional data related to Fig. 1 . (A and B) Expression of STIM1 and STIM2 in MDA-MB-231 (A) and LM2-4 (B) cells following CRISPR/Cas9 gene editing. Representative images from WB analysis of lysates prepared from the indicated MDA-MB-231 (A) or LM2-4 (B) cells using antibodies for STIM1, STIM2, actin, GAPDH, or tubulin. Clones F10 and E5 are shown here as additional controls and were not used in the present study. (C) Representative images (left) and quantitation (right) of adhesion assays (n = 5) using the indicated MDA-MB-231 cells. The field of view in each image measures 2 × 2 mm. (D and E) Representative images (left) and quantitation (right) of two invasion assays using WT, S1KO, S2KO, dKO (C4), or dKO(B6) MDA-MB-231 cells (D) or using dKO(B6) cells re-expressing EYFP-STIM1 or EYFP-STIM2 (E). Scale bar = 300 μm. Results from each experiment in C or in D were normalized to WT cells, while data from E were normalized to control dKO cells. Bars show the mean ± SEM. Statistics: one-way ANOVA with Tukey’s post hoc test; *P < 0.05, **P < 0.01, ***P < 0.001. Source data are available for this figure: SourceData FS1.