EAAT5 parameters and placement. (A) Comparison of ON rates for glutamate binding to EAAT5 (1 × 10⁶ to 1 × 10⁹ M/s). The black trace shows the average ± SD single-vesicle I_A(glu) in rods. (B) Illustration of EAAT5 (yellow puncta) placement in four different regions of the synaptic invagination: throughout the cleft (Cleft), adjacent to BP dendritic tips (BP tips), the neck of the invagination (Neck), and just outside the mouth of the invaginating synapse (Mouth). The ribbon is colored dark blue. HCs are shown in green and turquoise. RBPs are in red and purple. (C) Simulated EAAT5 anion channel activity after placing EAAT5 in the different regions shown in B with a glutamate ON-binding rate of 1 × 10⁸ M/s. (D) Simulated EAAT5 anion channel activity with placement in various locations using an ON rate of 1 × 10⁹ M/S. Traces show the average of 12 simulations run with different seed values.