The B cell mechanoresponse includes mechanosensing via the antigen receptor and adhesion molecules at the cell surface and mechanotransduction via the intracellular signaling pathways that convey mechanical information to the nucleus and cytoskeleton. This response manifests visibly on stiffer stimulatory substrates, with higher cell spreading observed during immunological synapse formation. Stiffer substrates, upon antigen receptor cross-linking, elicit higher cytoplasmic translocation of ATAT1, leading to an increase in acetylated microtubules. These modified microtubules dictate lysosomal and actin foci positioning at the center of the synapse, which further augments antigen uptake, processing, and presentation to T cells.