GS-expressing iCAF subsets co-localize with FOLR2 ⁺ macrophages in human breast cancer patients. (A) UMAP of 18,296 FAP⁺ CAFs from 7 breast cancer patients (Croizer et al., 2024) allowing for visualization of 8 CAF clusters, including 3 iCAFs clusters and 5 myCAF clusters. (B) UMAP showing GS gene expression in FAP⁺ CAF clusters. (C) Progeny pathway analysis in iCAF and myCAF subsets from scRNA-seq data. (D) Correlations of Detox-iCAF or IFNg-iCAF proportion and FOLR2⁺ macrophages in bulk RNA-seq data. (E) Correlation of number of Detox-iCAF or IFNg-iCAF and FOLR2⁺ macrophages per spot using spatial transcriptomics. P values from Pearson correlation test. (F) Graphical abstract demonstrating the hypothesis in this study on the triple cross talk between tumor cells, CAFs and macrophages.