Figure 5. NOMO is under single pN forces... | Rockefeller University Press

$NOMO is under single pN forces partly dependent on the Ig 1/10/11 interface. (A) Illustration of the TEV cleavage-based readout. (B) Representation of the 5 pN and 10 pN coiled-coil (cc) sensors and their location between Igs 11 and 12 in WT and 4-mutant NOMO. (C) Immunofluorescence images of WT or 4-Mut FLAG-NOMO in either the absence (−) or presence (+) of TEVp. (D) Representative immunoblot of indicated cc-force sensors inserted in WT and 4-mutant NOMO when TEVp is absent or present (+). (E) Quantification of fraction of the indicated FLAG-NOMO construct cleaved under the absence (−) and presence (+) of TEVp. N = 8. Each condition was measured from n = 6 biological replicates. Statistical analyses were performed using an ordinary one-way ANOVA test; ****P < 0.0001. Source numerical data, immunoblot replicates, and unprocessed blots are available in source data. Source data are available for this figure: SourceData F5.$

Figure 5.

NOMO is under single pN forces partly dependent on the Ig 1/10/11 interface. (A) Illustration of the TEV cleavage-based readout. (B) Representation of the 5 pN and 10 pN coiled-coil (cc) sensors and their location between Igs 11 and 12 in WT and 4-mutant NOMO. (C) Immunofluorescence images of WT or 4-Mut FLAG-NOMO in either the absence (−) or presence (+) of TEVp. (D) Representative immunoblot of indicated cc-force sensors inserted in WT and 4-mutant NOMO when TEVp is absent or present (+). (E) Quantification of fraction of the indicated FLAG-NOMO construct cleaved under the absence (−) and presence (+) of TEVp. N = 8. Each condition was measured from n = 6 biological replicates. Statistical analyses were performed using an ordinary one-way ANOVA test; ****P < 0.0001. Source numerical data, immunoblot replicates, and unprocessed blots are available in source data. Source data are available for this figure: SourceData F5.

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