scRNA-seq identifies heterogeneity among TAMs in Mycn-nGEMM tumors, which correlates with TAMs in human NB. (A) UMAP of 11,943 CD45⁺ cells and neuronal cells obtained from three replicates of Mycn-nGEMM tumors after integration. (B) Violin plot of Mif mRNA expression by cell type of the Mycn-nGEMM tumors. (C) UMAP of 1,780 Mycn-nGEMM TAMs. Three major clusters were identified and annotated according to marker genes and pathway analysis (see text for details). (D) Dotplot presenting the marker genes for each cluster of C. (E) Distributions of M2 signature (left) and M1 signature (right) scores for each TAM subtypes in C. (F) Heatmap of correlation scores between Mycn-nGEMM TAMs and human TAMs subtypes defined by Wienke et al. (2024). Columns represent Mycn-nGEMM TAM cells and rows represent human TAM subtypes. The colored bar at the top represents the Mycn-nGEMM macrophage clusters (subtypes).