PD-L1 expression in human MYCN-amplified NB and murine Mycn-nGEMM model. (A) Analysis of MYCN, PD-L1, MYC, and CD163 expression in a cohort of human neuroblastoma samples. (B) Heatmap representing expression of CD45, CD3e, PD-L1, GFP, and CD11b in Mycn-nGEMM tumors from Fig. 2. (C) Percentage of PD-L1⁺ cells in Phox2B⁺ and CD163⁺ population of individual samples included in Fig. 4 D. (D) PD-L1 expression assessed by flow cytometry on BMDM co-cultured with indicated conditioned media (Mycn-high, Mycn-low), treated or not with a MIF inhibitor (MIFi) or IFNγ for 72 h. Mean fluorescent intensity (MFI) for the PD-L1 signal is indicated for each condition.