Expression of Mycn in migrating mouse NCC leads to tumor formation in immunocompetent, syngeneic C57BL/6J hosts. (A) Sox10, Foxd3, and Ascl1 expression relative to HPRT as assessed by qRT-PCR in adrenal gland and NCC from E9.5 embryos. Data represent mean values ± SD from n = 3 independent experiments. (B) Hand2, Phox2a, Phox2b, and TH expression relative to HPRT as assessed by qRT-PCR in adrenal gland and NCC from E9.5 embryos. Data represent mean values ± SD from n = 2 independent experiments. (C)Mycn expression relative to HPRT as assessed by qRT-PCR in NCC-WT (non-transduced) and NCC-Mycn (transduced with the Mycn-GFP construct). Data represent mean values ± SD from n = 2 independent experiments. (D) N-Myc expression as assessed by western blot in NCC-WT (non-transduced) and NCC-Mycn (transduced with the Mycn-GFP construct). (E) Growth kinetics of NCC-WT (non-transduced) and NCC-Mycn (transduced with the Mycn-GFP construct) cells assessed by CTG assay. Data represent mean values ± SD of n = 3 independent experiments. (F)Mycn expression relative to HPRT as assessed by qRT-PCR in four cell lines derived from Mycn-nGEMM primary tumors. Data represent mean values ± SD from n = 2 independent experiments. (G) Growth kinetics of Mycn-nGEMM-high and Mycn-nGEMM-low cells assessed by CTG assay. Data represent mean values ± SD of n = 2 independent experiments. (H) Representative BLI images of Fig. 1 F. (I) Umap using GMKF dataset depending on risk. (J) Heatmap on copy number variation from Mycn-nGEMM tumors. Source data are available for this figure: SourceData FS1.