Organization of integrin receptors at the NMJ upon integrin-β neuronal downregulation. (A) Integrin-β (cyan) in axonal bundles (HRP in magenta) in control and larvae expressing the integrin-β/Mys³³⁶⁴² RNAi in neurons (C155-Gal4). (B) Quantification of axonal integrin-β fluorescence in CTRL³⁵⁷⁸⁵ vs Mys³³⁶⁴² neuronal RNAi. (C) Integrin-β (cyan) at NMJs (HRP in magenta) in control and integrin-β/Mys³³⁶⁴² animals. (D) Analyses of integrin-β fluorescence in HRP-delineated and postsynaptic areas, as well as in muscles of control and integrin-β/Mys³³⁶⁴² larvae. (E) Integrin-β WEKA-segmented particles in controls and integrin-β/Mys³³⁶⁴² larvae. (F) Analyses of spot-like integrin-β structures within the postsynaptic ROI. Control datasets are reused from Fig. 6. Box-and-whisker graphs were used to represent the results of the area and fluorescence intensity of the individual integrin-β particles. Whiskers represent 10^th to 90^th percentile, while the rest of the data point are shown as individual values. The Y axis in these graphs represents log₁₀, to capture the broad distribution of the individual values. In bar graphs with linear Y axis, error bars represent the SEM. N in bar graphs—NMJs; N in box and whiskers—individual integrin-β assemblies. Ns—nonsignificant, and ***P < 0.001 upon the unpaired, nonparametric Mann–Whitney test. Error bars are SEMs. Scale bar—2 µm. See Table S1 for detailed genotypes and N.