Neuronal depletion of nonmuscle myosin II rearranges integrin receptors at the NMJ. (A) Pearson’s R correlation between Lifeact::Halo and integrin-β::YFP at NMJs of live larvae. (B) Reorganization of integrin-β (cyan) at NMJs (HRP, magenta) of larvae expressing an independent RNAi line NMII^HC/Zip³⁶⁷²⁷ in neurons, compared with controls. (C) Fluorescence intensity per µm² of integrin-β within the HRP-delineated and postsynaptic areas in control and larvae expressing the independent Zip³⁶⁷²⁷. (D) Integrin-β (cyan) immunofluorescence at NMJs (HRP, magenta) in larvae carrying Zip⁶⁵⁹⁴⁷ in the absence and presence of C155-Gal4 driver. (E and F) Quantification of the fluorescence intensity per µm² of integrin-β within the HRP-delineated and postsynaptic areas in larvae carrying the CTRL³⁵⁷⁸⁵- and Zip⁶⁵⁹⁴⁷ RNAi lines, in the absence and presence of C155-Gal4. (G) Integrin-β immunofluorescence (cyan) at myotendinous junctions of larvae expressing CTRL and Zip⁶⁵⁹⁴⁷ in neurons. (H) Quantification of the fluorescence per µm² of integrin-β in myotendinous junctions in CTRL- and Zip⁶⁵⁹⁴⁷-expressing larvae (C155-Gal4>RNAi). Error bars represent the SEM. N—NMJs or myotendinous junctions, respectively. ns—nonsignificant, **P < 0.01, ***P < 0.001 upon the unpaired, nonparametric Mann–Whitney test. Scale bar—2 µm. See Table S1 for detailed genotypes and N.