Neuronal depletion of nonmuscle myosin II rearranges integrin receptors at the NMJ. (A) Distribution of YFP-tagged, ubiquitously expressed integrin-β (cyan) at NMJs in live larvae expressing Lifeact::Halo (magenta) in neurons (see also Video 9). White arrows point to areas of overlap between integrin-β and linear F-actin. (B) Immunostaining of integrin-β (cyan) at NMJs of control larvae. Yellow arrows depict integrin-β in linear structures, red arrows point to presynaptic spotlike integrin-β structures, while gray arrows point to postsynaptic spot-like integrin-β. (C) Reorganization of integrin-β at NMJs of larvae expressing NMII^HC/Zip⁶⁵⁹⁴⁷ and NMII^LC/Sqh³²⁴³⁹ in neurons, compared with controls. (D) Integrin-β fluorescence in the HRP-delineated, postsynaptic, and muscle ROIs. (E) Quantifications of the abundance, size, and fluorescence of WEKA-segmented linear integrin-β structures within the HRP-delineated area. (F) Analysis of the number, size, and fluorescence of WEKA-segmented integrin-β foci within the HRP-delineated area. (G) Analysis of the number, size, and fluorescence of WEKA-segmented postsynaptic integrin-β foci. Control datasets are also used in Fig. S10. Box-and-whisker graphs were used to represent the results of the area and fluorescence intensity of the individual integrin-β particles. Whiskers represent 10^th to 90^th percentile, while the rest of the data points are shown as individual values. The y axis in these graphs represents log₁₀, to capture the broad distribution of the individual values. In bar graphs with linear y axis, error bars represent the SEM. N in bar graphs—NMJs or muscle ROIs; N in box and whiskers—individual integrin-β assemblies. ns—nonsignificant, *P < 0.05, **P < 0.01, and ***P < 0.001 after one-way ANOVA with the Kruskal–Wallis multiple comparisons test. g—Hedges’ g represents the effect size. Scale bars—2 µm, inset in A with scale bar—1 µm. See Table S1 for detailed genotypes and N.