Figure 3.
NMII regulates the integrity of presynaptic actin assemblies. (A and B) QmNGMA-labeled presynaptic actin in larvae co-expressing CTRL35785 or (B) NMIIHC/Zip65947 RNAi (see also Videos 7 and 8). Representative NMJs are from independent larvae. (C) Quantification of fluorescence per µm2 for the QmNGMA actin marker in whole NMJs. (D) Quantifications of the number of round F-actin assemblies per µm2, as well as their area and fluorescence. (E) Graphs representing the percentage of NMJ area covered with linear F-actin, as well as the area and fluorescence of the individual assemblies. (F–H) Lifeact::Halo-labeled presynaptic F-actin in GFP-expressing control, or larvae expressing nonphosphorylatable SqhDN and constitutively phosphorylated SqhCA, respectively. (I) Quantification of fluorescence per µm2 for the Lifeact::Halo F-actin marker in whole NMJs. (J) Quantifications of the number of round F-actin assemblies per µm2, as well as their area and fluorescence. (K) Graphs representing the percentage of NMJ area covered with linear F-actin, as well as the area and fluorescence of the individual assemblies. Box-and-whisker graphs were used to represent the results of the area and fluorescence intensity of the individual presynaptic actin structures. Whiskers represent 10th to 90th percentile, while the rest of the data points are shown as individual values. The y axis in these graphs represents log10, to capture the broad distribution of the individual values. In bar graphs with linear y axis, error bars represent the SEM. N in bar graphs—NMJs; N in box and whiskers—individual actin assemblies. ns—not significant, *P < 0.05, **P < 0.01, and ***P < 0.001 upon the unpaired, nonparametric Mann–Whitney test. g–Hedges’ g represents the effect size. Scale bars—2 µm. See Table S1 for detailed genotypes and N.

NMII regulates the integrity of presynaptic actin assemblies. (A and B) QmNGMA-labeled presynaptic actin in larvae co-expressing CTRL35785 or (B) NMIIHC/Zip65947 RNAi (see also Videos 7 and 8). Representative NMJs are from independent larvae. (C) Quantification of fluorescence per µm2 for the QmNGMA actin marker in whole NMJs. (D) Quantifications of the number of round F-actin assemblies per µm2, as well as their area and fluorescence. (E) Graphs representing the percentage of NMJ area covered with linear F-actin, as well as the area and fluorescence of the individual assemblies. (F–H) Lifeact::Halo-labeled presynaptic F-actin in GFP-expressing control, or larvae expressing nonphosphorylatable SqhDN and constitutively phosphorylated SqhCA, respectively. (I) Quantification of fluorescence per µm2 for the Lifeact::Halo F-actin marker in whole NMJs. (J) Quantifications of the number of round F-actin assemblies per µm2, as well as their area and fluorescence. (K) Graphs representing the percentage of NMJ area covered with linear F-actin, as well as the area and fluorescence of the individual assemblies. Box-and-whisker graphs were used to represent the results of the area and fluorescence intensity of the individual presynaptic actin structures. Whiskers represent 10th to 90th percentile, while the rest of the data points are shown as individual values. The y axis in these graphs represents log10, to capture the broad distribution of the individual values. In bar graphs with linear y axis, error bars represent the SEM. N in bar graphs—NMJs; N in box and whiskers—individual actin assemblies. ns—not significant, *P < 0.05, **P < 0.01, and ***P < 0.001 upon the unpaired, nonparametric Mann–Whitney test. g–Hedges’ g represents the effect size. Scale bars—2 µm. See Table S1 for detailed genotypes and N.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal