Dysregulation of cholesterol biosynthesis in iNgly1 ^−/− mouse cerebellum. (A and B) Pathway enrichment analysis of DEGs between Ngly1^fl/fl and iNgly1^−/− astrocytes and oligodendrocytes. (C and D) Fluorescent IHC staining of HMGCR in 8-wk-old Ngly1^fl/fl and iNgly1^−/− cerebella. Scale bar, 20 μm. Fluorescence intensity per Bergmann glia of >130 cells from four mice per genotype was quantified and shown in D. Unpaired Student’s t test. ***P < 0.001. (E and F) Dot plots of cholesterol metabolism–related genes in Ngly1^fl/fl, iNgly1^−/−, and iNgly1^−/−Sting1^−/− astrocytes and oligodendrocytes (ODC). (G) Quantification of Ngly1^fl/fl, iNgly1^−/−, and iNgly1^−/−Sting1^−/− (three genotypes in each cluster).