snRNA-seq analysis of cells in iNgly1 ^−/− mouse cerebellum. (A and B) Dot plots of proteasome subunit and autophagy/mitophagy-related gene expression in Ngly1^fl/fl, iNgly1^−/−, and iNgly1^−/−Sting1^−/− mouse microglia. (C and D) Fluorescent IHC staining of PSMB2 and TAX1BP1 in 8-wk-old Ngly1^fl/fl, iNgly1^−/− cerebella. Scale bar, 20 μm. Fluorescence intensity per Purkinje cell of >200 cells from four mice per genotype was quantified and is shown on the right. Unpaired Student’s t test. ***P < 0.001. (E) Violin plots of ISG gene expression in Ngly1^fl/fl, iNgly1^−/−, and iNgly1^−/−Sting1^−/− mouse microglia.