snRNA-seq analysis of cells in iNgly1 ^−/− mouse cerebellum. (A) Experimental workflow of snRNA-seq of 8-wk-old Ngly1^fl/fl, iNgly1^−/−, and iNgly1^−/−Sting1^−/− mouse cerebella (n = 2 mice per genotype). (B) UMAP plot of all cells from iNgly1^−/−, iNgly1^−/−Sting1^−/−, and Ngly1^fl/fl mouse cerebella. (C) Dot plots of cell type–specific marker genes. (D and E) Dot plots of proteasome subunit and autophagy/mitophagy-related genes in Ngly1^fl/fl, iNgly1^−/−, and iNgly1^−/−Sting1^−/− Purkinje cells. (F) Pathway enrichment analysis of DEGs between Ngly1^fl/fl and iNgly1^−/− Bergmann glia. (G) Dot plots of cholesterol metabolism–related genes in Ngly1^fl/fl, iNgly1^−/−, and iNgly1^−/−Sting1^−/− Bergmann glia. (H) UMAP plot of granule cell subcluster from Ngly1^fl/fl, iNgly1^−/−, or iNgly1^−/−Sting1^−/− mouse cerebella. (I) Quantification of three genotypes in each granule cell subcluster. (J) Dot plots of hallmark gene expression in GC1 and GC2 subclusters. UMAP, Uniform Manifold Approximation and Projection.