Deletion of Ngly1 in Purkinje cells or microglia alone did not cause neurodegeneration. (A) Representative IHC staining of Purkinje cell marker calbindin in cerebella of 6-mo-old Ngly1^fl/fl, Ngly1^fl/flPcp2-cre, and Ngly1^fl/flCx3cr1-cre control mice. Scale bar, 1 mm (upper panel), 100 μm (lower panel). (B) Quantification of calbindin-immunoreactive Purkinje cells shown in A. Data are shown as the mean ± SEM. Unpaired Student’s t test. ns, not significant.